Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.
Identifieur interne : 000362 ( Main/Exploration ); précédent : 000361; suivant : 000363Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.
Auteurs : Iman R. Abdel-Shafi [Égypte] ; Eman Y. Shoieb [Égypte] ; Samar S. Attia [Égypte] ; José M. Rubio [Espagne] ; Thuy-Huong Ta-Tang [Espagne] ; Ayman A. El-Badry [Égypte]Source :
- Parasitology research [ 1432-1955 ] ; 2017.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Alignement de séquences, Animaux, Culex (génétique), Données de séquences moléculaires, Enfant, Enfant d'âge préscolaire, Femelle, Filariose lymphatique (parasitologie), Filariose lymphatique (sang), Filariose lymphatique (épidémiologie), Humains, Jeune adulte, Mâle, Nourrisson, Phylogénie, Réaction de polymérisation en chaîne, Sang (parasitologie), Sujet âgé, Sujet âgé de 80 ans ou plus, Séquence nucléotidique, Wuchereria bancrofti (), Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification), Égypte (épidémiologie).
- MESH :
- génétique : Culex, Wuchereria bancrofti.
- isolement et purification : Wuchereria bancrofti.
- parasitologie : Filariose lymphatique, Sang.
- sang : Filariose lymphatique.
- épidémiologie : Filariose lymphatique, Égypte.
- Adolescent, Adulte, Adulte d'âge moyen, Alignement de séquences, Animaux, Données de séquences moléculaires, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Nourrisson, Phylogénie, Réaction de polymérisation en chaîne, Sujet âgé, Sujet âgé de 80 ans ou plus, Séquence nucléotidique, Wuchereria bancrofti.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Base Sequence, Blood (parasitology), Child, Child, Preschool, Culex (genetics), Egypt (epidemiology), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (parasitology), Female, Humans, Infant, Male, Middle Aged, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Wuchereria bancrofti (classification), Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification), Young Adult.
- MESH :
- blood : Elephantiasis, Filarial.
- classification : Wuchereria bancrofti.
- epidemiology : Egypt, Elephantiasis, Filarial.
- genetics : Culex, Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- parasitology : Blood, Elephantiasis, Filarial.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Base Sequence, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Young Adult.
Abstract
Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.
DOI: 10.1007/s00436-017-5372-7
PubMed: 28120100
Affiliations:
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Le document en format XML
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Abdel-Shafi</name><affiliation wicri:level="1"><nlm:affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo</wicri:regionArea><wicri:noRegion>Cairo</wicri:noRegion></affiliation></author><author><name sortKey="Shoieb, Eman Y" sort="Shoieb, Eman Y" uniqKey="Shoieb E" first="Eman Y" last="Shoieb">Eman Y. 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type="abstract" xml:lang="en">Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>Égypte</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Égypte"><noRegion><name sortKey="Abdel Shafi, Iman R" sort="Abdel Shafi, Iman R" uniqKey="Abdel Shafi I" first="Iman R" last="Abdel-Shafi">Iman R. Abdel-Shafi</name></noRegion><name sortKey="Attia, Samar S" sort="Attia, Samar S" uniqKey="Attia S" first="Samar S" last="Attia">Samar S. Attia</name><name sortKey="El Badry, Ayman A" sort="El Badry, Ayman A" uniqKey="El Badry A" first="Ayman A" last="El-Badry">Ayman A. El-Badry</name><name sortKey="Shoieb, Eman Y" sort="Shoieb, Eman Y" uniqKey="Shoieb E" first="Eman Y" last="Shoieb">Eman Y. Shoieb</name></country><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Rubio, Jose M" sort="Rubio, Jose M" uniqKey="Rubio J" first="José M" last="Rubio">José M. Rubio</name></region><name sortKey="Ta Tang, Thuy Huong" sort="Ta Tang, Thuy Huong" uniqKey="Ta Tang T" first="Thuy-Huong" last="Ta-Tang">Thuy-Huong Ta-Tang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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